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Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μg/ml.
Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.
Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 μg/mL.
Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 μg/mL.<br><br>Green: HES5 Antibody (8055) <br> Blue: DAPI staining
Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.
Trial Size

HES5 Antibody

CATALOG NUMBER: 8055

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.
The immunogen is located within the first 50 amino acids of HES5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 18 kDa
Observed: 17 kDa
Purification:
HES5 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HES5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HES5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
HES5
Additional Names:
Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38
Protein Accession Number:
NP_001010926
Protein Gi Number:
58219048
Ncbi Gene Id Number:
388585
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).
Background Reference 1:
Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34.
Background Reference 2:
Karlsson C, Jonsson M, Asp J, et al. Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 2007; 327:539-51.
Background Reference 3:
Sancho R, Blake SM, Tendeng C, et al. Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions. PLoS Biol. 2013; 11:e1001586.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8055

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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