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45-727 (0.1µg/ml) staining of Human (A) and Mouse (B) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
45-727 (0.1µg/ml) staining of Human (A) and Mouse (B) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

HEXIM1 Antibody

CATALOG NUMBER: 45-727

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Immunogen:
The immunogen for this antibody is: C-HRQQERAPLSKFGD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human and Mouse Heart lysates (calculated MW of 40.6kDa according to Human NP_006451.1 and 40.2kDa according to Mouse NP_620092.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperatur. Immunofluorescence: Strong expression of the protein seen in the nucleus of A431 and HeLa cells. Recommended concentration: 10µg/ml.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HEXIM1
Additional Names:
HEXIM1, hexamethylene bis-acetamide inducible 1, HGNC:24953, CLP1, HIS1, MAQ1 , HMBA-inducible, hexamethylene bisacetamide-inducible protein, hexamethylene-bis-acetamide-inducible transcript 1, hexamthylene bis-acetamide inducible 1, menage a quatre 1, EDG1, FLJ13562
Protein Accession Number:
NP_006451.1
Protein Gi Number:
5453682
Ncbi Gene Id Number:
10614
Background Reference 1:
Schulte A, Czudnochowski N, Barboric M, Schonichen A, Blazek D, Peterlin BM, Geyer M. Identification of a Cyclin T-Binding Domain in Hexim1 and Biochemical Analysis of Its Binding Competition with HIV-1 Tat. J Biol Chem. 2005 Jul 1;280(26):24968-77. Epub 2005 Apr 25.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-727

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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