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Western blot analysis of extracts of various cell lines, using Hexokinase II antibody (13-360) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using Hexokinase II antibody (13-360). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Hexokinase II antibody (13-360) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Hexokinase II Antibody

CATALOG NUMBER: 13-360

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
K-562
Positive Control 3:
HL-60
Predicted Molecular Weight:
Observed: 102kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HK2
Additional Names:
HK2, DKFZp686M1669, HKII, HXK2
Ncbi Gene Id Number:
3099
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-360

List Size:
100 uL

List Price:

$436.00

Datasheet
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