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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

HEYL Antibody

CATALOG NUMBER: 58-862

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HEYL
Additional Names:
Hairy/enhancer-of-split related with YRPW motif-like protein, hHeyL, Class B basic helix-loop-helix protein 33, bHLHb33, Hairy-related transcription factor 3, HRT-3, hHRT3, HEYL, BHLHB33, HRT3
Protein Accession Number:
Q9NQ87
Protein Gi Number:
146286205
Ncbi Gene Id Number:
26508
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the hairy and enhancer ofsplit-related (HESR) family of basic helix-loop-helix (bHLH)-typetranscription factors. The sequence of the encoded protein containsa conserved bHLH and orange domain, but its YRPW motif has divergedfrom other HESR family members. It is thought to be an effector ofNotch signaling and a regulator of cell fate decisions.Alternatively spliced transcript variants have been found, buttheir biological validity has not been determined. [provided byRefSeq].
Background Reference 1:
Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005)
Background Reference 2:
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Background Reference 3:
Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003)
Background Reference 4:
Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-862

List Size:
400 ul

List Price:

$495.00

Datasheet
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