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Western blot analysis of lysates from human skeletal muscle and heart tissue lysates (from left to right), using HFE2 Antibody at 1:1000 at each lane.
HFE2 Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of lysates from human skeletal muscle and heart tissue lysates (from left to right), using HFE2 Antibody at 1:1000 at each lane.

HFE2 Antibody

CATALOG NUMBER: 64-070

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-162 amino acids from the Central region of human HFE2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HFE2
Additional Names:
Hemojuvelin, Hemochromatosis type 2 protein, RGM domain family member C, HFE2, HJV, RGMC
Protein Accession Number:
Q6ZVN8
Protein Gi Number:
51316254
Ncbi Gene Id Number:
148738
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HFE2 is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin.
Background Reference 1:
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Background Reference 2:
Barton, J.C., et al. Am. J. Hematol. 84(11):710-714(2009)
Background Reference 3:
Zhang, A.S., et al. J. Biol. Chem. 284(34):22580-22589(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-070

List Size:
400 ul

List Price:

$495.00

Datasheet
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