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Western blot analysis of hIMOS-1-Q271 in mouse spleen tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of hIMOS-1-Q271 in mouse spleen tissue lysates (35ug/lane)

HGS Antibody

CATALOG NUMBER: 61-121

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This HGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the N-terminal region of human HGS.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
86 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HGS
Additional Names:
Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110, HGS, HRS
Protein Accession Number:
O14964
Ncbi Gene Id Number:
9146
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. HGS may concentrate ubiquitinated receptors within clathrin-coated regions. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.
Background Reference 1:
Kirk E, et al. Cell Sci. 2006 Nov 15;119(Pt 22):4689-701
Background Reference 2:
Sasaki Y, et al. Biol Chem. 2001 Aug 10;276(32):29943-52.
Background Reference 3:
Miura S, et al. Mol Cell Biol. 2000 Dec;20(24):9346-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-121

List Size:
400 ul

List Price:

$495.00

Datasheet
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