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Western blot analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 μg/mL.
Immunohistochemistry of HHATL in human skin tissue with HHATL antibody at 2.5 μg/mL.
Immunofluorescence of HHATL in human skin tissue with HHATL antibody at 20 μg/mL.
Western blot analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 μg/mL.
Trial Size

HHATL Antibody

CATALOG NUMBER: 6787

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HHATL antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HHATL.
The immunogen is located within the first 50 amino acids of HHATL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
HHATL antibody can be used for detection of HHATL by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 12-701 - Human Skin Tissue Slide
Purification:
HHATL Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HHATL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HHATL antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HHATL
Additional Names:
HHATL Antibody: GUP1, OACT3, C3orf3, MBOAT3, MSTP002, GUP1, KIAA1173, Protein-cysteine N-palmitoyltransferase HHAT-like protein, Hedgehog acyltransferase-like protein
Protein Accession Number:
NP_065758
Protein Gi Number:
239582769
Ncbi Gene Id Number:
57467
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HHATL Antibody: The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development.
Background Reference 1:
Abe Y, Kita Y, and Niikura T. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-palmitoylation of Sonic hedgehog. FEBS J. 2008; 275:318-31.
Background Reference 2:
Zhang SQ, Tian X, Luo YW, et al. Expression, clinical and pathological significance of KIAA1173 gene in skin squamous cell carcinoma. Zhonghua Yi Xue Za Zhi 2010; 90:1243-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6787

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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