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HEK293 overexpressing Human HIC1 and probed with 43-339 (0.5ug/ml) with the mock transfection in second lane. Data obtained from Dr. D. Leprince, CNRS UMR 8161, Institut de Biologie de LILLE, France
43-339 (1ug/ml) staining of A549 (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Human HIC1 and probed with 43-339 (0.5ug/ml) with the mock transfection in second lane. Data obtained from Dr. D. Leprince, CNRS UMR 8161, Institut de Biologie de LILLE, France

HIC1 Antibody

CATALOG NUMBER: 43-339

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: NTHCAELYASGP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kDa is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kDa according to NP_006488.2. Recommended concentration, 0.5-1ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HIC1
Additional Names:
HIC1, hypermethylated in cancer 1, ZBTB29, ZNF901, hic-1, hypermethylated in cancer 1 protein, zinc finger and BTB domain-containing protein 29
Protein Accession Number:
NP_006488.2,NP_001091672.1
Protein Gi Number:
61676186
Ncbi Gene Id Number:
3090
Background Reference 1:
Yu J, Liu P, Cui X, Sui Y, Ji G, Guan R, Sun D, Ji W, Liu F, Liu A, Zhao Y, Yu Y, Jin Y, Bai J, Geng J, Xue Y, Qi J, Lee KY, Fu S.Identification of novel subregions of LOH in gastric cancer and analysis of the HIC1 and TOB1 tumor suppressor genes in these subregions.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-339

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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