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Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2(0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).
Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2(0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).
HIF1Alpha Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from Raji cell line, untreated or treated with CoCl2(0. 1mM), using HIF1Alpha Antibody (upper) or Beta-actin (lower).

HIF1Alpha Antibody

CATALOG NUMBER: 63-185

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine
Immunogen:
This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:250
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIF1A
Additional Names:
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
Protein Accession Number:
Q16665
Protein Gi Number:
2498017
Ncbi Gene Id Number:
3091
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
Background Reference 1:
Lee, M.N., et al. J. Natl. Cancer Inst. 102(6):426-442(2010) Mayer, A., et al. Adv. Exp. Med. Biol. 662, 399-405 (2010)Brouwer, E., et al. Clin. Exp. Rheumatol. 27(6):945-951(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-185

List Size:
400 ul

List Price:

$495.00

Datasheet
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