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Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 μg/mL.
Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

HINT1 Antibody

CATALOG NUMBER: 4815

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
Immunogen:
HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1.
The immunogen is located within the last 50 amino acids of HINT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Purification:
HINT1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HINT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HINT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HINT1
Additional Names:
HINT1 Antibody: HINT, NMAN, PKCI-1, PRKCNH1, HINT, PKCI1, Histidine triad nucleotide-binding protein 1, Protein kinase C inhibitor 1
Protein Accession Number:
NP_005331
Protein Gi Number:
4885413
Ncbi Gene Id Number:
3094
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HINT1 Antibody: Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.
Background Reference 1:
Lima CD, Klein MG, Weinstein IB, et al. Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins. Proc. Natl. Acad. Sci. USA1996; 93:5357-62.
Background Reference 2:
Weiske J and Huber O. Beta-catenin takes a HIT. Cell Cycle2008; 7:1326-31.
Background Reference 3:
Wang L, Zhang Y, Li H, et al. Hint1 inhibits growth and activator protein-1 activity in human colon cancer cells. Cancer Res.2007; 67:4700-8.
Background Reference 4:
Chen Q, Wang X, O'Neill FA, et al. Is the histidine triad nucleotide-binding protein 1 (HINT1) gene a candidate for schizophrenia? Schizophr. Res.2008; 106:200-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4815

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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