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45-730 (0.03ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-730 (0.03ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

HIP-55 Antibody

CATALOG NUMBER: 45-730

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: AANLSRNGPALQE-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 48.3kDa according to NP_054782.2). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_054782.2; NP_001014436.1; NP_001116428.1)
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
DBNL
Additional Names:
DBNL, HIP-55, SH3P7, src homology 3 domain-containing protein HIP-55, HIP-55 protein, drebrin F, drebrin-like, ABP1
Protein Accession Number:
NP_054782.2,NP_001014436.1,NP_001116428.1
Protein Gi Number:
21361670
Ncbi Gene Id Number:
28988
Background Reference 1:
Ensenat D, Yao Z, Wang XS, Kori R, Zhou G, Lee SC, Tan TH. A novel src homology 3 domain-containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1. J Biol Chem. 1999 Nov 26;274(48):33945-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-730

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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