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Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (22-631) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ZNF148 antibody (22-622) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (22-631).
Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (22-631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

HIRIP3 Antibody

CATALOG NUMBER: 22-631

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
HL-60
Positive Control 2:
HeLa
Positive Control 3:
U-937
Positive Control 4:
Jurkat
Positive Control 5:
Mouse skeletal muscle
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 62kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HIRIP3
Additional Names:
HIRA-interacting protein 3, HIRIP3
Ncbi Gene Id Number:
8479
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-631

List Size:
100 uL

List Price:

$436.00

Datasheet
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