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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
HIST1H1D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

HIST1H1D Antibody

CATALOG NUMBER: 57-240

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HIST1H1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human HIST1H1D.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIST1H1D
Additional Names:
Histone H13, Histone H1c, Histone H1s-2, HIST1H1D, H1F3
Protein Accession Number:
P16402
Protein Gi Number:
121925
Ncbi Gene Id Number:
3007
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins responsible fornucleosome structure of the chromosomal fiber in eukaryotes. Twomolecules of each of the four core histones (H2A, H2B, H3, and H4)form an octamer, around which approximately 146 bp of DNA iswrapped in repeating units, called nucleosomes. The linker histone,H1, interacts with linker DNA between nucleosomes and functions inthe compaction of chromatin into higher order structures. This geneis intronless and encodes a member of the histone H1 family.Transcripts from this gene lack polyA tails but instead contain apalindromic termination element. This gene is found in the largehistone gene cluster on chromosome 6.
Background Reference 1:
Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010)
Background Reference 2:
Soranzo, N., et al. PLoS Genet.(4), E1000445 (2009) :
Background Reference 3:
Sovio, U., et al. PLoS Genet.(3), E1000409 (2009) :
Background Reference 4:
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-240

List Size:
400 ul

List Price:

$495.00

Datasheet
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