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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: L929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody . was diluted at 1:1000 at each lane.
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: L929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.

HIST1H2AG Antibody

CATALOG NUMBER: 61-041

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila
Immunogen:
This HIST1H2AG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-87 amino acids from the Central region of human HIST1H2AG.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:25
For FACS starting dilution is: 1:25
Predicted Molecular Weight:
14 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIST1H2AG
Additional Names:
Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP
Protein Accession Number:
P0C0S8
Protein Gi Number:
83288406
Ncbi Gene Id Number:
83,298,330,833,283,300,000
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background Reference 1:
Albig W.,et al.Hum. Genet. 101:284-294(1997).
Background Reference 2:
Albig W.,et al.Biol. Chem. 380:7-18(1999).
Background Reference 3:
Dobner T.,et al.DNA Seq. 1:409-413(1991).
Background Reference 4:
Mannironi C.,et al.DNA Cell Biol. 13:161-170(1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-041

List Size:
400 ul

List Price:

$469.00

Out of stock

Datasheet
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