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Western blot analysis in HL-60,HepG2,K562,CEM,MCF-7 cell line lysates (35ug/lane).
HIST1H2AL Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60,HepG2,K562,CEM,MCF-7 cell line lysates (35ug/lane).

HIST1H2AL Antibody

CATALOG NUMBER: 61-701

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine, Mouse
Immunogen:
This HIST1H2AL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the C-terminal region of human HIST1H2AL.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
14 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIST1H2AG
Additional Names:
Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP
Protein Accession Number:
P0C0S8
Protein Gi Number:
83288406
Ncbi Gene Id Number:
8.32983E+19
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Background Reference 1:
Lusic, M., et al. EMBO J. 22(24):6550-6561(2003)
Background Reference 2:
Kzhyshkowska, J., et al. Biochem. J. 371 (PT 2), 385-393 (2003)
Background Reference 3:
Marzluff, W.F., et al. Genomics 80(5):487-498(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-701

List Size:
400 ul

List Price:

$495.00

Datasheet
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