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Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HIST1H2BH/HIST1H2BK/HIST3H2BB antibody detected the HIST1H2BH/HIST1H2BK/HIST3H2BB protein (arrow).
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HIST1H2BH/HIST1H2BK/HIST3H2BB antibody detected the HIST1H2BH/HIST1H2BK/HIST3H2BB protein (arrow).

HIST1H2BH/HIST1H2BK/HIST3H2BB Antibody

CATALOG NUMBER: 59-972

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Mouse, Bovine, Chicken, Zebrafish, Rat
Immunogen:
This HIST1H2BH/HIST1H2BK/HIST3H2BB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-52 amino acids from the Central region of human HIST1H2BH/HIST1H2BK/HIST3H2BB.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
14 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIST1H2BH
Additional Names:
Histone H2B type 1-H, Histone H2Bj, H2B/j, HIST1H2BH, H2BFJ
Protein Accession Number:
Q93079
Protein Gi Number:
7387739
Ncbi Gene Id Number:
8345
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsiblefor the nucleosome structure of the chromosomal fiber ineukaryotes. Two molecules of each of the four core histones (H2A,H2B, H3, and H4) form an octamer, around which approximately 146 bpof DNA is wrapped in repeating units, called nucleosomes. Thelinker histone, H1, interacts with linker DNA between nucleosomesand functions in the compaction of chromatin into higher orderstructures. This gene is intronless and encodes a member of thehistone H2B family. Transcripts from this gene lack polyA tails butinstead contain a palindromic termination element. This gene isfound in the large histone gene cluster on chromosome 6. [providedby RefSeq].
Background Reference 1:
Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
Background Reference 2:
Beck, H.C., et al. Mol. Cell Proteomics 5(7):1314-1325(2006)
Background Reference 3:
Pavri, R., et al. Cell 125(4):703-717(2006)
Background Reference 4:
Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-972

List Size:
400 ul

List Price:

$495.00

Datasheet
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