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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

HIST2H3A Antibody

CATALOG NUMBER: 60-649

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat, Chicken, Zebrafish, Xenopus, C. Elegans, Drosophila, Pig, Rabbit
Immunogen:
This HIST2H3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the C-terminal region of human HIST2H3A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
15 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIST2H3A
Additional Names:
Histone H32, Histone H3/m, Histone H3/o, HIST2H3A
Protein Accession Number:
Q71DI3
Protein Gi Number:
74758899
Ncbi Gene Id Number:
1.26961E+17
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsiblefor the nucleosome structure of the chromosomal fiber ineukaryotes. This structure consists of approximately 146 bp of DNAwrapped around a nucleosome, an octamer composed of pairs of eachof the four core histones (H2A, H2B, H3, and H4). The chromatinfiber is further compacted through the interaction of a linkerhistone, H1, with the DNA between the nucleosomes to form higherorder chromatin structures. This gene is intronless and encodes amember of the histone H3 family. Transcripts from this gene lackpolyA tails; instead, they contain a palindromic terminationelement. This gene is found in a histone cluster on chromosome 1.This gene is one of four histone genes in the cluster that areduplicated; this record represents the telomeric copy. [provided byRefSeq].
Background Reference 1:
Neumann, H., et al. Mol. Cell 36(1):153-163(2009)
Background Reference 2:
Hurd, P.J., et al. J. Biol. Chem. 284(24):16575-16583(2009)
Background Reference 3:
Yuan, J., et al. Cell Cycle 8(11):1747-1753(2009)
Background Reference 4:
Chang, Q., et al. J. Hepatol. 50(2):323-333(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-649

List Size:
400 ul

List Price:

$495.00

Datasheet
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