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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

HIST3H3 Antibody

CATALOG NUMBER: 56-287

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HIST3H3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the Central region of human HIST3H3.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HIST3H3
Additional Names:
Histone H31t, H3/t, H3t, H3/g, HIST3H3, H3FT
Protein Accession Number:
Q16695
Protein Gi Number:
18202512
Ncbi Gene Id Number:
8290
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsiblefor the nucleosome structure of the chromosomal fiber ineukaryotes. Nucleosomes consist of approximately 146 bp of DNAwrapped around a histone octamer composed of pairs of each of thefour core histones (H2A, H2B, H3, and H4). The chromatin fiber isfurther compacted through the interaction of a linker histone, H1,with the DNA between the nucleosomes to form higher order chromatinstructures. This gene is intronless and encodes a member of thehistone H3 family. Transcripts from this gene lack polyA tails;instead, they contain a palindromic termination element. This geneis located separately from the other H3 genes that are in thehistone gene cluster on chromosome 6p22-p21.3. [provided byRefSeq].
Background Reference 1:
Tachiwana, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10454-10459(2010)
Background Reference 2:
Nair, S.S., et al. EMBO Rep. 11(6):438-444(2010)
Background Reference 3:
Rampakakis, E., et al. J. Cell. Biochem. 108(2):400-407(2009)
Background Reference 4:
Mochizuki, K., et al. Biochem. Biophys. Res. Commun. 371(2):324-327(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-287

List Size:
400 ul

List Price:

$495.00

Datasheet
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