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45-732 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-732 (4ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
45-732 (0.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Histamine Receptor H1 Antibody

CATALOG NUMBER: 45-732

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: QQTSVRREDKCETD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Heart lysates (calculated MW of 55.8kDa according to NP_000852.1). Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli. Recommended concentration, 2-4ug/ml.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HRH1
Additional Names:
histamine receptor H1, H1-R, HRH1, hisH1 , histamine receptor, subclass H1
Protein Accession Number:
NP_000852.1
Protein Gi Number:
4504491
Ncbi Gene Id Number:
3269
Background Reference 1:
Bryce PJ, Mathias CB, Harrison KL, Watanabe T, Geha RS, Oettgen HC. The H1 histamine receptor regulates allergic lung responses. J Clin Invest. 2006 Jun;116(6):1624-32. Epub 2006 May 4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-732

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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