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Western blot analysis of lysed extracts from 293 cells using Histone H3.1 (Ab-10).
Immunofluorescence staining of methanol-fixed HeLa cells using Histone H3.1 (Ab-10).
Western blot analysis of lysed extracts from 293 cells using Histone H3.1 (Ab-10).

Histone H3.1 (Ab 10) Antibody

CATALOG NUMBER: 79-555

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Histone H3.1 (Ab-10) antibody was raised against a peptide sequence around aa.8~12 (R-K-S-T-G) derived from Human Histone H3.1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total Histone H3.1 protein.
Predicted Molecular Weight:
17 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HIST1H3D
Additional Names:
H3/b, H3FB, H3/a, H3/c, H3/d, H3/f, H3/h
Protein Accession Number:
NP_003521.2
Protein Gi Number:
21071021
Ncbi Gene Id Number:
8351
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background Reference 1:
Workman, J. L. and Kingston, R. E. (1998) Annu Rev Biochem 67, 545-79. Hansen, J. C. et al. (1998) Biochemistry 37, 17637-41. Strahl, B. D. and Allis, C. D. (2000) Nature 403, 41-5. Cheung, P. et al. (2000) Cell 103, 263-71.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-555

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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