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<strong>Figure 1 Western Blot Validation with HIV-1 p24 Protein </strong><br>Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7367, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.
<strong>Figure 2 ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein </strong><br>Coating Antigen: HIV-1 p24 recombinant protein, 1 μg/mL, incubated at 4 °C overnight. Detection Antibodies: HIV-1 p24 antibody, PM-7367, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.
Figure 1 Western Blot Validation with HIV-1 p24 Protein
Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, PM-7367, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5,000 dilution.
New
Trial Size

HIV-1 p24 Antibody [8D7E11] (HRP)

CATALOG NUMBER: PM-7367-HRP

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Virus
Conjugate:
HRP
Specifications
Host Species:
Mouse
Species Reactivity:
Virus
Immunogen:
Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
Conjugate:
HRP
Tested Applications:
ELISA, WB
Application Note:
WB: 1 μg/mL.
Anti-HIV-1 p24 antibody detects HIV-1 p24 protein by ELISA and WB. All other applications and species not yet tested.
Predicted Molecular Weight:
24 kDa
Purification:
Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HIV-1 p24 Monoclonal Antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GAG
Additional Names:
Anti-HIV-1 p24 antibody: Pr55Gag, Gag polyprotein, Capsid protein p24
Protein Accession Number:
AAB50258
Protein Gi Number:
327745
Ncbi Gene Id Number:
155030
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core, and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 protein has been used as an integral part of multicomponent HIV-1 vaccines (3).
Background Reference 1:
Goto et al. Micron 1998; 29:123-38.
Background Reference 2:
Freed EO. Virology 1998; 251:1-15.
Background Reference 3:
Freed EO. Virology 1998; 251:1-15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-7367-HRP

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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