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<strong>Figure 1 ELISA Validation of HIV-1 p24 Recombinant Protein </strong><br>Coating Antigen: HIV-1 p24 protein, 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: HIV-1 p24 antibodies, PM-6335, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
<strong>Figure 2 SDS-PAGE Validation of HIV-1 p24 Recombinant Protein </strong><br>Loading: 0.5 μg (lane 1) and 1 μg (lane 2) of HIV-1 p24 recombinant protein, 95-130.Observed: 24kD
<strong>Figure 3 WB Validation of HIV-1 p24 Protein with HIV-1 p24 Antibodies </strong><br>Loading: 5 ng, 10 ng and 25 ng of HIV-1 p24 protein. Detection Antibodies: HIV-1 p24 antibody, PM-6335, 0.5 μg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.
Figure 1 ELISA Validation of HIV-1 p24 Recombinant Protein
Coating Antigen: HIV-1 p24 protein, 1 μg/mL, incubated at 4 °C overnight.Detection Antibodies: HIV-1 p24 antibodies, PM-6335, dilution: 0.3- 1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr.

HIV-1 p24 Recombinant Protein

CATALOG NUMBER: 95-130

Tested Applications:
ELISA, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Virus
source-species:
E. coli
Recombinant Protein Sequence:
Full length of HIV-1 Gag p24 protein
Fusion Tag:
His tag at C-terminus
Tested Applications:
ELISA, SDS-PAGE, WB
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Predicted Molecular Weight:
24 kD
Purity:
> 95% as determined by Bis-Tris PAGE
physical-state:
Liquid
Buffer:
PBS (pH 7.4).
Storage Conditions:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
gag
Additional Names:
Gag polyprotein, Pr55Gag, MA, Caspid p24
Protein Accession Number:
AAB50258
Ncbi Gene Id Number:
155030
Background:
HIV-1 p24 Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix. The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein. The p24 is the major capsid protein of the virus and has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different strains.
Background Reference 1:
Goto et al. Micron 1998; 29:123-38.
Background Reference 2:
Freed. Virology 1998; 251:1-15.
Background Reference 3:
Lindenburg et al. Vaccine 2002; 20 :2343-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-130

List Size:
0.05 mg

List Price:

$289.00

Datasheet
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