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Western blot analysis in Jurkat cell line lysates (35ug/lane).
HJURP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

HJURP Antibody

CATALOG NUMBER: 57-033

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HJURP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 559-588 amino acids from the C-terminal region of human HJURP.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HJURP
Additional Names:
Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9, HJURP
Protein Accession Number:
Q8NCD3
Protein Gi Number:
239938642
Ncbi Gene Id Number:
55355
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HJURP is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
Background Reference 1:
Sebastiani, P., et al. Science (2010) In press :
Background Reference 2:
Shuaib, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1349-1354(2010)
Background Reference 3:
Hu, Z., et al. Breast Cancer Res. 12 (2), R18 (2010) :
Background Reference 4:
Dunleavy, E.M., et al. Cell 137(3):485-497(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-033

List Size:
400 ul

List Price:

$495.00

Datasheet
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