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Western blot analysis of extracts of various cell lines, using HK1 antibody (13-849).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using HK1 antibody (13-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using HK1 antibody (13-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using HK1 antibody (13-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using HK1 antibody (13-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse skin using HK1 antibody (13-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse colon using HK1 antibody (13-849) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HK1 antibody (13-849).
Immunofluorescence analysis of MCF-7 cells using HK1 antibody (13-849).
Western blot analysis of extracts of various cell lines, using HK1 antibody (13-849).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HK1 Antibody [D0F7]

CATALOG NUMBER: 13-849

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human HK1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
293T
Positive Control 3:
MCF7
Positive Control 4:
HepG2
Predicted Molecular Weight:
Observed: 102kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HK1
Additional Names:
HK, HXK
Ncbi Gene Id Number:
3098
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-849

List Size:
50 uL

List Price:

$335.00

Datasheet
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