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Western Blot at 1:4000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of HLA-DPB1 using rabbit polyclonal HLA-DPB1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DPB1 gene.
HLA-DPB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:4000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.

HLA-DPB1 Antibody

CATALOG NUMBER: 57-302

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HLA-DPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the Central region of human HLA-DPB1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HLA-DPB1
Additional Names:
HLA class II histocompatibility antigen, DP beta 1 chain, HLA class II histocompatibility antigen, DP(W4) beta chain, MHC class II antigen DPB1, HLA-DPB1, HLA-DP1B
Protein Accession Number:
P04440
Protein Gi Number:
122263
Ncbi Gene Id Number:
3115
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HLA-DPB belongs to the HLA class II beta chain paralogues.This class II molecule is a heterodimer consisting of an alpha(DPA) and a beta chain (DPB), both anchored in the membrane. Itplays a central role in the immune system by presenting peptidesderived from extracellular proteins. Class II molecules areexpressed in antigen presenting cells (APC: B lymphocytes,dendritic cells, macrophages). The beta chain is approximately26-28 kDa and its gene contains 6 exons. Exon one encodes theleader peptide, exons 2 and 3 encode the two extracellular domains,exon 4 encodes the transmembrane domain and exon 5 encodes thecytoplasmic tail. Within the DP molecule both the alpha chain andthe beta chain contain the polymorphisms specifying the peptidebinding specificities, resulting in up to 4 different molecules.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Yang, J.H., et al. Tissue Antigens 76(4):282-288(2010)
Background Reference 3:
Wang, P., et al. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24(6):261-263(2010)
Background Reference 4:
Ovsyannikova, I.G., et al. PLoS ONE(7), E11806 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-302

List Size:
400 ul

List Price:

$495.00

Datasheet
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