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Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
HLA-DRB5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

HLA-DRB5 Antibody

CATALOG NUMBER: 64-133

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HLA-DRB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the Central region of human HLA-DRB5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HLA-DRB5
Additional Names:
HLA class II histocompatibility antigen, DR beta 5 chain, DR beta-5, DR2-beta-2, Dw2, MHC class II antigen DRB5, HLA-DRB5 {ECO:0000312, EMBL:CAI180791}
Protein Accession Number:
Q30154
Protein Gi Number:
74754558
Ncbi Gene Id Number:
3127
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
Background Reference 1:
Asano, K., et al. Nat. Genet. 41(12):1325-1329(2009)
Background Reference 2:
Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009)
Background Reference 3:
Montano-Loza, A.J., et al. Liver Int. 29(10):1543-1551(2009)
Background Reference 4:
Lincoln, M.R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(18):7542-7547(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-133

List Size:
400 ul

List Price:

$495.00

Datasheet
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