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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

HLF Antibody

CATALOG NUMBER: 59-187

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HLF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human HLF.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HLF
Additional Names:
Hepatic leukemia factor, HLF
Protein Accession Number:
Q16534
Protein Gi Number:
6919907
Ncbi Gene Id Number:
3131
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the proline and acidic-rich(PAR) protein family, a subset of the bZIP transcription factors.The encoded protein forms homodimers or heterodimers with other PARfamily members and binds sequence-specific promoter elements toactivate transcription. Chromosomal translocations fusing portionsof this gene with the E2A gene cause a subset of childhoodB-lineage acute lymphoid leukemias. Alternatively splicedtranscript variants have been described, but their biologicalvalidity has not been determined.
Background Reference 1:
Hirose, K., et al. Blood 116(6):962-970(2010)
Background Reference 2:
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Background Reference 3:
Otto, T., et al. Endocrinology 149(5):2241-2250(2008)
Background Reference 4:
Inukai, T., et al. Leukemia 21(2):288-296(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-187

List Size:
400 ul

List Price:

$495.00

Datasheet
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