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Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in WB on Human Jurkat 2.5 ug/ml.

HMBOX1 Antibody

CATALOG NUMBER: 29-090

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMBOX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HMBOX1 antibody can be used for detection of HMBOX1 by ELISA at 1:312500. HMBOX1 antibody can be used for detection of HMBOX1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HMBOX1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HMBOX1
Additional Names:
HMBOX1, HOT1, PBHNF, HNF1LA
Protein Accession Number:
NP_078843
Protein Gi Number:
47059040
Ncbi Gene Id Number:
79618
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HMBOX1 is located at the boundary of 8p12.3 and 8p21.1. HMBOX1 proteins are highly conserved in human, mouse, rat, chicken and Xenopus laevis. Functional HMBOX1::EGFP (enhanced green fluorescent protein) fusion protein revealed that HMBOX1 accumulated more in cytoplasm than in nucleus. HMBOX1 is a transcription repressor. Human HMBOX1 is widely expressed in 18 tissues, and it is highly expressed in pancreas. Hmbox1 is widely expressed in mouse pancreas and the expression of this gene can also be detected in pallium, hippocampus and hypothalamus.
Background Reference 1:
Strausberg, R.L., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-090

List Size:
100 ul

List Price:

$473.00

Datasheet
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