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Western blot analysis of extracts of various cell lines, using HMG20B antibody (19-360) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using HMG20B antibody (19-360) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

HMG20B Antibody

CATALOG NUMBER: 19-360

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human HMG20B (NP_006330.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
SKOV3
Positive Control 4:
Mouse kidney
Positive Control 5:
Mouse uterus
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HMG20B
Additional Names:
BRAF25, BRAF35, HMGX2, HMGXB2, PP7706, SMARCE1r, SOXL, pp8857, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related, BRCA2-associated factor 35, HMG box domain containing 2, HMG box-containing protein 20B, HMG domain-containing protein 2, HMG domain-containing protein HMGX2, SMARCE1-related protein, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related, Sox-like transcriptional factor, structural DNA-binding protein BRAF35
Ncbi Gene Id Number:
10362
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-360

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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