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42-115 (0.5ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
42-115 (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-115 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-115 (0.5ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

HMGA1 Antibody

CATALOG NUMBER: 42-115

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow, Pig
Immunogen:
The immunogen for this antibody is: C-SQPLASKQEKDGT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 24kDa band observed in Human Pancreas (calculated MW of 11.7kDa/10.7kDa according to NP_665906.1; NP_002122.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Placenta. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HMGA1
Additional Names:
HMGA1, high mobility group AT-hook 1, HMG-R, HMGA1A, HMGIY, MGC12816, MGC4242, MGC4854, OTTHUMP00000016222, OTTHUMP00000016223, OTTHUMP00000016224, OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y
Protein Accession Number:
NP_665906.1,NP_002122.1
Protein Gi Number:
22208967
Ncbi Gene Id Number:
3159
Background Reference 1:
Liau SS, Rocha F, Matros E, Redston M, Whang E, High mobility group AT-hook 1 (HMGA1) is an independent prognostic factor and novel therapeutic target in pancreatic adenocarcinoma. Cancer 2008 Jul 113 (2): 302-14.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-115

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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