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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

HMGB1 Antibody

CATALOG NUMBER: 28-791

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGB1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
HMGB1 antibody can be used for detection of HMGB1 by ELISA at 1:62500. HMGB1 antibody can be used for detection of HMGB1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
25 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store HMGB1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
HMGB1
Additional Names:
HMGB1, DKFZp686A04236, HMG1, HMG3, SBP-1
Protein Accession Number:
NP_002119
Protein Gi Number:
4504425
Ncbi Gene Id Number:
3146
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Extracellular HMGB1 is an activator of human tumor cell migration operating in concert with EGF. HMGB1 encodes a protein that is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription.
Background Reference 1:
Bruneel, A., (2005) Proteomics 5 (15), 3876-3884.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-791

List Size:
100 ul

List Price:

$473.00

Datasheet
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