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Western blot analysis of extracts of various cell lines, using HMGB1 antibody (18-706) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (18-706) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (18-706) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HMGB1 antibody (18-706) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HMGB1 antibody (18-706) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
KO

HMGB1 Antibody, KO Validated

CATALOG NUMBER: 18-706

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
MCF7
Positive Control 3:
Jurkat
Positive Control 4:
Mouse spleen
Positive Control 5:
Mouse lung
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 29kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HMGB1
Additional Names:
HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1
Ncbi Gene Id Number:
3146
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-706

List Size:
100 uL

List Price:

$436.00

Datasheet
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