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46-979 (0.01ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-979 (0.01ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HMGB3 Antibody

CATALOG NUMBER: 46-979

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Cow
Immunogen:
The immunogen for this antibody is: C-KFDGAKGPAKVARKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 24kDa band observed in Human Lung lysates (calculated MW of 23.0kDa according to NP_005333.2). Recommended concentration: 0.01-0.03ug/ml.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HMGB3
Additional Names:
HMGB3, high-mobility group box 3, HMG2A, HMG4, MGC90319, high-mobility group (nonhistone chromosomal) protein 4, non-histone chromosomal protein
Protein Accession Number:
NP_005333.2
Protein Gi Number:
71143137
Ncbi Gene Id Number:
3149
Background Reference 1:
Yu F, Yao H, Zhu P, Zhang X, Pan Q, Gong C, Huang Y, Hu X, Su F, Lieberman J, Song E. let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell. 2007 Dec 14;131(6):1109-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-979

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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