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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

HMGB3 Antibody

CATALOG NUMBER: 57-896

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse
Immunogen:
This HMGB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the Central region of human HMGB3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HMGB3
Additional Names:
High mobility group protein B3, High mobility group protein 2a, HMG-2a, High mobility group protein 4, HMG-4, HMGB3, HMG2A, HMG4
Protein Accession Number:
O15347
Ncbi Gene Id Number:
3149
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HMGB3 belongs to the high mobility group (HMG) proteinsuperfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3contains DNA-binding HMG box domains and is classified into the HMGbox subfamily. Members of the HMG box subfamily are thought to playa fundamental role in DNA replication, nucleosome assembly andtranscription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al.,2006 [PubMed 16945912]).
Background Reference 1:
Nemeth, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(37):13783-13788(2006)
Background Reference 2:
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Background Reference 3:
Vaccari, T., et al. Genomics 49(2):247-252(1998)
Background Reference 4:
Wilke, K., et al. Gene 198 (1-2), 269-274 (1997) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-896

List Size:
400 ul

List Price:

$495.00

Datasheet
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