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Western blot analysis of HMGCS1 antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of hela cells using HMGCS1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HMGCS1 antibody in K562 cell line lysates (35ug/lane)

HMGCS1 Antibody

CATALOG NUMBER: 62-381

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Hamster, Mouse, Rat
Immunogen:
This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-470 amino acids from the C-terminal region of human HMGCS1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HMGCS1
Additional Names:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS1, HMGCS
Protein Accession Number:
Q01581
Protein Gi Number:
1708239
Ncbi Gene Id Number:
3157
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HMGCS1 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Background Reference 1:
Vock,C., Cell. Physiol. Biochem. 22 (5-6), 515-524 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-381

List Size:
400 ul

List Price:

$495.00

Datasheet
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