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43-134 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-134 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HMGI-C / HMGA2 Antibody

CATALOG NUMBER: 43-134

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-KAAQKKAEATGEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 18kDa band observed in Human Heart lysates (calculated MW of 11.8kDa according to NP_003474.1 and 11.5kDa according to NP_003475.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Di Cello et al, Mol Cancer Res. 2008 May;6(5):743-50.; PMID: 18505920). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003474.1; NP_003475.1) and is not expected to cross-react with HMGA1.
Predicted Molecular Weight:
Approx 18kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HMGI-C / HMGA2
Additional Names:
HMGA2, high mobility group AT-hook 2, BABL, HMGI-C, HMGIC, LIPO, STQTL9, High-mobility group protein HMGI-C, high-mobility group (nonhistone chromosomal) protein isoform I-C
Protein Accession Number:
NP_003474.1,NP_003475.1
Protein Gi Number:
4504431
Ncbi Gene Id Number:
8091
Background Reference 1:
Patel UA, Bandiera A, Manfioletti G, Giancotti V, Chau KY, Crane-Robinson CExpression and cDNA cloning of human HMGI-C phosphoprotein

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-134

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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