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Western blot analysis of HMHA1 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lymph reacted with HMHA1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells using HMHA1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HMHA1 Antibody in A2058 cell line lysates (35ug/lane)

HMHA1 Antibody

CATALOG NUMBER: 62-570

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HMHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 877-903 amino acids from the Central region of human HMHA1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
125 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HMHA1
Additional Names:
Minor histocompatibility protein HA-1, Minor histocompatibility antigen HA-1, mHag HA-1, HMHA1, KIAA0223
Protein Accession Number:
Q92619
Protein Gi Number:
187471158
Ncbi Gene Id Number:
23526
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The minor histocompatibility antigen (mHags), HA-1 is a immunogenic alloantigen shown to be responsible for graft-versus-host disease (GVHD) in HLA-identical bone marrow transplantation. The antigen has two known alleles resulting in a single amino acid polymorphism. The HA-1H allele encodes histidine, whereas the HA-1R allele encodes arginine.
Background Reference 1:
Gillespie,G.,et.al., Hematol. J.(6), 403-410 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-570

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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