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Western blot analysis of HMOX1 using rabbit polyclonal HMOX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMOX1 gene.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HMOX1 using rabbit polyclonal HMOX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HMOX1 gene.

HMOX1 Antibody

CATALOG NUMBER: 55-728

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This HMOX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-204 amino acids from the Central region of human HMOX1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HMOX1
Additional Names:
Heme oxygenase 1, HO-1, HMOX1, HO, HO1
Protein Accession Number:
P09601
Protein Gi Number:
123446
Ncbi Gene Id Number:
3162
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Heme oxygenase, an essential enzyme in heme catabolism,cleaves heme to form biliverdin, which is subsequently converted tobilirubin by biliverdin reductase, and carbon monoxide, a putativeneurotransmitter. Heme oxygenase activity is induced by itssubstrate heme and by various nonheme substances. Heme oxygenaseoccurs as 2 isozymes, an inducible heme oxygenase-1 and aconstitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the hemeoxygenase family.
Background Reference 1:
Wu, M.M., et al. Toxicol. Appl. Pharmacol. 248(3):226-233(2010)
Background Reference 2:
Bolisetty, S., et al. J. Am. Soc. Nephrol. 21(10):1702-1712(2010)
Background Reference 3:
Bao, W., et al. PLoS ONE(8), E12371 (2010) :
Background Reference 4:
Wu, M.M., et al. J. Biomed. Sci. 17, 70 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-728

List Size:
400 ul

List Price:

$495.00

Datasheet
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