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Western blot analysis of HNF1A Antibody in mouse liver tissue lysates (35ug/lane)
Confocal immunofluorescent analysis of HNF1A Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of HNF1A Antibody in mouse liver tissue lysates (35ug/lane)

HNF1A Antibody

CATALOG NUMBER: 63-609

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This HNF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-205 amino acids from the Central region of human HNF1A.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
67 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HNF1A
Additional Names:
Hepatocyte nuclear factor 1-alpha, HNF-1-alpha, HNF-1A, Liver-specific transcription factor LF-B1, LFB1, Transcription factor 1, TCF-1, HNF1A, TCF1
Protein Accession Number:
P20823
Protein Gi Number:
51338763
Ncbi Gene Id Number:
6927
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HNF1A is a transcription factor required for the expression of several liver-specific genes. This protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Background Reference 1:
Bach,I., et.al., Genomics(1), 155-164 (1990)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-609

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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