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Western blot analysis of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35ug/lane)
Western blot analysis of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35ug/lane)

HNF4A Antibody

CATALOG NUMBER: 61-499

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Xenopus
Immunogen:
This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-146 amino acids from human HNF4A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HNF4A
Additional Names:
Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14
Protein Accession Number:
P41235
Protein Gi Number:
148886624
Ncbi Gene Id Number:
3172
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HNF4A is a nuclear transcription factor which binds DNA as a homodimer. This protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This protein may play a role in development of the liver, kidney, and intestines. Mutations in this protein have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.
Background Reference 1:
Sugai,M., Pathol. Int. 58 (11), 681-686 (2008)
Background Reference 2:
Lee,S.S., Hepatology 48 (2), 635-645 (2008)
Background Reference 3:
Guo,H., Biochem. J. 394 (PT 2), 379-387 (2006)
Background Reference 4:
Hong,Y.H., J. Biol. Chem. 278 (30), 27495-27501 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-499

List Size:
400 ul

List Price:

$495.00

Datasheet
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