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Western blot analysis in 293,HepG2 cell line and rat liver tissue lysates (35ug/lane).
HNF4A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of HNF4A Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in 293,HepG2 cell line and rat liver tissue lysates (35ug/lane).

HNF4A Antibody

CATALOG NUMBER: 61-500

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-312 amino acids from the Central region of human HNF4A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HNF4A
Additional Names:
Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, HNF4A, HNF4, NR2A1, TCF14
Protein Accession Number:
P41235
Protein Gi Number:
148886624
Ncbi Gene Id Number:
3172
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HNF4A is a nuclear transcription factor which binds DNA as a homodimer. This protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This protein may play a role in development of the liver, kidney, and intestines. Mutations in HNF4A gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.
Background Reference 1:
Kritis A.A., Argyrokastritis A.Gene 173:275-280(1996)
Background Reference 2:
Yamagata K., Furuta H., Oda N.Nature 384:458-460(1996)
Background Reference 3:
Furuta H., Iwasaki N., Oda N.Diabetes 46:1652-1657(1997)
Background Reference 4:
Moeller A.M., Urhammer S.A.Diabetologia 40:980-983(1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-500

List Size:
400 ul

List Price:

$495.00

Datasheet
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