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Western blot analysis of various lysates using hnRNP A1 Rabbit mAb at 1：1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Western blot analysis of various lysates using hnRNP A1 Rabbit mAb at 1：1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human colon using hnRNP A1 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Confocal imaging of C6 cells using hnRNP A1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red).The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of U-2 OS cells using hnRNP A1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red).The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using hnRNP A1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red).The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg hnRNP A1 antibody. Western blot was performed from the immunoprecipitate using hnRNP A1 antibody at a dilution of 1:1000.
Western blot analysis of various lysates using hnRNP A1 Rabbit mAb at 1：1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

hnRNP A1 Rabbit Recombinant Antibody (81-184)

CATALOG NUMBER: 81-184

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Application Note:
WB,1:1000 - 1:2000, IHC-P,1:100 - 1:1000, IF/ICC,1:200 - 1:2000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa,293T,HepG2,Mouse brain,Rat brain
Predicted Molecular Weight:
39kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HNRNPA1
Additional Names:
UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
Protein Accession Number:
P09651
Ncbi Gene Id Number:
3178
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.hnRNP A1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-184

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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