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Western blot analysis of extracts of various cell lines, using HNRNPA1 antibody (22-980) at 1:200 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5min.
Immunohistochemistry of paraffin-embedded rat spinal cord using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain astrocytoma using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HNRNPA1 Antibody (22-980) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 antibody (22-816) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37 for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using HNRNPA1 antibody (22-980).
Western blot analysis of extracts of various cell lines, using HNRNPA1 antibody (22-980) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.

HNRNPA1 Antibody

CATALOG NUMBER: 22-980

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human HNRNPA1 (NP_112420.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
Jurkat
Positive Control 3:
HepG2
Positive Control 4:
HL-60
Positive Control 5:
293T
Predicted Molecular Weight:
Observed: 39kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HNRNPA1
Additional Names:
HNRPA1, ALS19, ALS20, HNRPA1, IBMPFD3, HNRPA1L3, hnRNP A1, hnRNP-A1
Ncbi Gene Id Number:
3178
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-980

List Size:
100 uL

List Price:

$436.00

Datasheet
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