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43-499 (0.3ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-499 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-499 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-499 (0.1ug/ml) staining of NIH3T3 (A), (0.01ug/ml) nuclear NIH3T3 (B) lysate, + Peptide (C) (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-499 (0.3ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

HNRNPA2B1 Antibody

CATALOG NUMBER: 43-499

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DTIEIITDRQS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line MCF7 and approx. 35kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0kDa according to NP_002128.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.01-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_002128.1; NP_112533.1).
Predicted Molecular Weight:
Approx 36kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HNRNPA2B1
Additional Names:
HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, RNPA2, SNRPB1, heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2 / hnRNP B1, nuclear ribonucleoprotein particle A2 protein
Protein Accession Number:
NP_002128.1,NP_112533.1
Protein Gi Number:
14043072
Ncbi Gene Id Number:
3181
Background Reference 1:
Pastor T, Pagani F.Interaction of hnRNPA1/A2 and DAZAP1 with an Alu-derived intronic splicing enhancer regulates ATM aberrant splicing.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-499

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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