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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293T,A431,NCI-H292,Raji cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with HNRNPD Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.

HNRNPD Antibody

CATALOG NUMBER: 58-256

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HNRNPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the N-terminal region of human HNRNPD.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HNRNPD
Additional Names:
Heterogeneous nuclear ribonucleoprotein D0, hnRNP D0, AU-rich element RNA-binding protein 1, HNRNPD, AUF1, HNRPD
Protein Accession Number:
Q14103
Protein Gi Number:
13124489
Ncbi Gene Id Number:
3184
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the subfamily of ubiquitouslyexpressed heterogeneous nuclear ribonucleoproteins (hnRNPs). ThehnRNPs are nucleic acid binding proteins and they complex withheterogeneous nuclear RNA (hnRNA). These proteins are associatedwith pre-mRNAs in the nucleus and appear to influence pre-mRNAprocessing and other aspects of mRNA metabolism and transport.While all of the hnRNPs are present in the nucleus, some seem toshuttle between the nucleus and the cytoplasm. The hnRNP proteinshave distinct nucleic acid binding properties. The protein encodedby this gene has two repeats of quasi-RRM domains that bind toRNAs. It localizes to both the nucleus and the cytoplasm. Thisprotein is implicated in the regulation of mRNA stability.Alternative splicing of this gene results in four transcriptvariants.
Background Reference 1:
Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010)
Background Reference 2:
Zhai, B., et al. J. Biol. Chem. 285(31):23568-23580(2010)
Background Reference 3:
Vazquez-Chantada, M., et al. Gastroenterology 138(5):1943-1953(2010)
Background Reference 4:
Trojanowicz, B., et al. Endocr. Relat. Cancer 16(3):857-871(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-256

List Size:
400 ul

List Price:

$495.00

Datasheet
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