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Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.
Immunohistochemistry of HOOK3 in mouse brain tissue with HOOK3 antibody at 10 μg/ml.
Immunofluorescence of HOOK3 in mouse brain tissue with HOOK3 antibody at 20 μg/ml.<br><br>Red: HOOK3 Antibody (8425) <br> Blue: DAPI staining
Western blot analysis of HOOK3 in mouse brain tissue lysate with HOOK3 antibody at 1 μg/ml.
Trial Size

HOOK3 Antibody

CATALOG NUMBER: 8425

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3.
The immunogen is located within amino acids 210 - 260 of HOOK3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 79 kDa
Observed: 77 kDa
Purification:
HOOK3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HOOK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HOOK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
HOOK3
Additional Names:
hook microtubule-tethering protein 3, HK3, h-hook3, hHK3, FLJ31058, Protein Hook homolog 3
Protein Accession Number:
NP_115786
Protein Gi Number:
14165274
Ncbi Gene Id Number:
84376
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).
Background Reference 1:
Walenta JH, Didier AJ, Liu X, et al. The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins. J. Cell Biol. 2001; 152:923-34.
Background Reference 2:
Sano H, Ishino M, Krämer H, et al. The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. J. Biol. Chem. 2007; 282:7973-81.
Background Reference 3:
Herrmann L, Wiegmann C, Arsalan-Werner A, et al. Hook proteins: association with Alzheimer pathology and regulatory role of hook3 in amyloid beta generation. PLoS One 2015; 10:e0119423.
Background Reference 4:
Ciampi R, Giordano TJ, Wikenheiser-Brokamp K, et al. HOOK3-RET: a novel type of RET/PTC rearrangement in papillary thyroid carcinoma. Endocr. Relat. Cancer 2007; 14:445-52.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8425

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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