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Western blot analysis of HOPX in human brain tissue lysate with HOPX antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of HOPX in human heart tissue with HOPX antibody at 5 μg/mL.
Immunofluorescence of HOPX in human heart tissue with HOPX antibody at 20 μg/mL.
Western blot analysis of HOPX in human brain tissue lysate with HOPX antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

HOPX Antibody

CATALOG NUMBER: 8143

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (88%), Mouse: (88%), Bovine: (88%), Pig: (81%), Chicken: (81%)
Immunogen:
HOPX antibody was raised against a 16 amino acid peptide near the amino terminus of human HOPX.
The immunogen is located within the first 50 amino acids of HOPX.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
HOPX antibody can be used for detection of HOPX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
HOPX antibody is human specific. At least three isoforms of HOPX are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Predicted Molecular Weight:
Predicted: 12 kDa
Observed: 18 kDa
Purification:
HOPX antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HOPX antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HOPX antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
HOPX
Additional Names:
HOP homeobox, homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO
Protein Accession Number:
NP_001138932
Protein Gi Number:
224451025
Ncbi Gene Id Number:
84525
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HOPX is a small homeodomain protein that lacks normally conserved residues required for DNA binding (1). It is thought to act downstream of NKX2-5 and modulates serum response factor (SRF)-dependent cardiac-specific gene expression and cardiac development (1). HOPX also acts a tumor suppressor gene in multiple tumors and cancer cell lines (2,3). HOPX has been reported to regulate the proliferation/differentiation homeostasis in different cell types, including keratinocytes (4).
Background Reference 1:
Chen F, Kook H, Milewski R, et al. Hop is an unusual homeobox gene that modulates cardiac development. Cell 2002; 110:713-23.
Background Reference 2:
Asanoma K, Matsuda T, Kondo H, et al. NECC1, a candidate choriocarcinoma suppressor gene that encodes a homeodomain consensus motif. Genomics 2003; 81:15-25.
Background Reference 3:
Katoh H, Yamashita K, Waraya M, et al. Epigenetic silencing of HOPX promotes cancer progression in colorectal cancer. Neoplasia 2012; 14:559-71.
Background Reference 4:
Obarzanek-Fojt M, Favre B, Kypriotou M, et al. Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes. Eur. J. Cell Biol. 2011; 90:279-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8143

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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