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43-398 (0.2ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-398 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-398 (0.3ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-398 (0.2ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

HOXA10 Antibody

CATALOG NUMBER: 43-398

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: PQATSCSFAQNIKE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37-38kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 42.4kDa according to Human NP_061824.3 and 43.2kDa according to Mouse NP_032289.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining preferentially in the spermatocytes and spermatids. Recommended concentration, 5-10ug/ml.
Predicted Molecular Weight:
Approx 38kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HOXA10
Additional Names:
HOXA10, homeobox A10, HOX1, HOX1.8, HOX1H, PL, homeo box A10, homeobox protein 1H, homeobox protein HOXA10, homeobox protein Hox-1.8, homeobox protein Hox-1H, homeobox protein Hox-A10
Protein Accession Number:
NP_061824.3
Protein Gi Number:
182765442
Ncbi Gene Id Number:
3206
Background Reference 1:
Petracco R, Grechukhina O, Popkhadze S, Massasa E, Zhou Y, Taylor HS.MicroRNA 135 regulates HOXA10 expression in endometriosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-398

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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