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Western blot analysis of HOXA3 Antibody in HL-60 cell line lysates (35ug/lane)
HOXA3 Antibody IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of HOXA3 Antibody in HL-60 cell line lysates (35ug/lane)

HOXA3 Antibody

CATALOG NUMBER: 64-170

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Zebrafish
Immunogen:
This HOXA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 413-440 amino acids from the C-terminal region of human HOXA3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HOXA3
Additional Names:
Homeobox protein Hox-A3, Homeobox protein Hox-1E, HOXA3, HOX1E
Protein Accession Number:
O43365
Ncbi Gene Id Number:
3200
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation.
Background Reference 1:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Background Reference 2:
Chung, N., et al. Mol. Biol. Rep. 36(2):227-235(2009)
Background Reference 3:
Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006)
Background Reference 4:
Manzanares, M., et al. EMBO J. 21(3):365-376(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-170

List Size:
400 ul

List Price:

$495.00

Datasheet
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