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Western blot analysis of in mouse kidney tissue lysates (35ug/lane)
Flow cytometric analysis of 293 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of in mouse kidney tissue lysates (35ug/lane)

HOXA5 Antibody

CATALOG NUMBER: 62-408

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Drosophila, Zebrafish, Chicken, Bovine, Rat, Xenopus
Immunogen:
This HOXA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-223 amino acids from the C-terminal region of human HOXA5.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
HOXA5
Additional Names:
Homeobox protein Hox-A5, Homeobox protein Hox-1C, HOXA5, HOX1C
Protein Accession Number:
P20719
Protein Gi Number:
52788238
Ncbi Gene Id Number:
3202
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA5 gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis.
Background Reference 1:
Chen,Y., Blood 111 (3), 1217-1226 (2008)
Background Reference 2:
Chen,H., Cancer Res. 67 (17), 8007-8013 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-408

List Size:
400 ul

List Price:

$495.00

Datasheet
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